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Fig. 6 | Molecular Medicine

Fig. 6

From: Targeting the eCIRP/TREM-1 interaction with a small molecule inhibitor improves cardiac dysfunction in neonatal sepsis

Fig. 6

The eCIRP/TREM-1 interaction increases inflammation in cardiomyocytes. Primary murine neonatal cardiomyocytes were isolated and treated with rmCIRP for 24 h. a TREM-1 expression was assessed by RT-PCR (N = 6/group). Cardiomyocytes were treated with rmCIRP for 24 h with or without 20-min pretreatment with 10 µg/mL M3. rmCIRP stimulation increased levels of b IL-6 and c TNF-⍺ in the supernatant. M3 mitigated this inflammatory response. The last column shows M3 treatment alone as a control. Data are result of 2 independent experiments and expressed as means ± SE and compared by Kruskal–Wallis test with Dunn method (*p < 0.05 vs. PBS control and #p < 0.05 vs. rmCIRP-treated cells; PBS = 12, rmCIRP = 15, rmCIRP + M3 = 15, and M3 alone = 5)

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Molecular Medicine

ISSN: 1528-3658