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Fig. 7 | Molecular Medicine

Fig. 7

From: Vagal afferent fibers contribute to the anti-inflammatory reactions by vagus nerve stimulation in concanavalin A model of hepatitis in rats

Fig. 7

Downregulation of ChAT production by CAP. a, b Western blotting of ChAT for the lysates from DVC areas prepared from individual groups as indicated in the figure. AP/CN; a group injected with AP-5/CNQX into the DMV. Keta/Xyla; ketamine and xylazine. Western blot images in a and b are the representatives from 4 independent experiments. c, d RT-PCR for ChAT and AChE mRNAs. Amplified DNAs were analyzed by DNA gel electrophoresis and identified in all groups as a single band at 529 bp for ChAT and 307 bp for AChE. RT-PCR for actin as loading control. In a–d, one way ANOVA with Tukey post hoc is shown. ***p < 0.001 (n = 4)

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Molecular Medicine

ISSN: 1528-3658