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Fig. 4 | Molecular Medicine

Fig. 4

From: Early detection of steroid-induced femoral head necrosis using 99mTc-Cys-Annexin V-based apoptosis imaging in a rabbit model

Fig. 4

Apoptosis was detected via TUNEL assay with a fluorescence microscope (× 200). There was no abnormal apoptosis detected in control group at any of the time points (images a). TUNEL staining showed gradual increases in apoptosis in model group (images bd). Scale bar = 50 um. The apoptosis index in model group was significantly higher than that in control group at each time point (images e). DAPI wss used to mark all cells, blue dots in the figure, and TUNEL wss used to stain apoptotic cells, green dots in the figure) (*compared with control group, P < 0.05. The red arrow shows the TUNEL-positive cells). Model group vs control group: the 2nd week (n = 9 vs 8), 4th week(n = 8 vs 8), and 6th week (n = 9 vs 8)

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