Skip to main content
Fig. 2 | Molecular Medicine

Fig. 2

From: microRNA-199a-5p regulates epithelial-to-mesenchymal transition in diabetic cataract by targeting SP1 gene

Fig. 2

SP1 was the direct target of miR-199a. a Bioinformatics-based target analysis showed that SP1 is a potential target of miR-199a. b Luciferase reporter assay showed that the luciferase activity of SP1 3′UTR-wt significantly decreased with miR-199a mimic transfection, comparing to that of the NC mimic or SP1 3′UTR-mutant group (n = 3). c–e In the HG-treated cells, the mRNA and protein expression of SP1 increased significantly compared to NG control cells (n = 9). f The level of miR-199a was increased with miR-199a mimic transfection and decreased in the inhibitor group, compared with the normal cells, or negative control mimic/inhibitor. Meanwhile, lipofectamine (Lipo Ctr) did not affect miR-199a expression (n = 3). g–i SP1 mRNA and protein levels were downregulated significantly with the miR-199a mimic transfection, while they were upregulated when miR-199a was inhibited (n = 9). SP1 specific protein 1, HG high glucose, Lipo Ctr only lipofectamine treated as the lipofectamine control. *P < 0.05

Back to article page