Fig. 3

Up-regulation of ROCK1 in the lens epithelial cells from age-related cataract patients and the old mice respectively. a Up-regulation of ROCK1 in LECs from cataract patients compared to the control group. Lens sections were immunostained to assess the ROCK1 expression in the LECs from the patient and control group. Scale bar: 20 µm. b Western blotting estimated that the protein level of ROCK1 between the patient and the control group. The 100–250 kDa blot was retained by horizontal shear and incubated with the ROCK1 antibody. The 35–50 kDa blot was retained by horizontal shear and incubated with the β-actin antibody. c Lens sections were immunostained to assess the ROCK1 expression in the LECs from young and old mice, Scale bar: 20 µm. d Western blotting estimated that the protein level of ROCK1 between young and old mice, β-actin was used as an internal reference control. The 100–250 kDa blot was retained by horizontal shear and incubated with the ROCK1 antibody. The 35–50 kDa blot was retained by horizontal shear and incubated with the β-actin antibody