Fig. 4

Up-regulation of ROCK1 in apoptotic epithelial cells in response to H₂O₂. a H₂O₂ inhibited the viability of LECs as measured by the MTT assays. B3 cells were incubated in the absence or presence of different doses of H₂O₂ (100 and 200 µM) for 24 h, and cell viability was assessed by measuring absorbance at 492 nm with a microplate reader. **p < 0.01 versus the untreated cells. b The apoptosis detection of LECs under different doses of H₂O₂ (100 and 200 µM) treatment for 24 h by Hoechst 33342 staining assay. The red arrow showed the nuclear morphology stained with blue by using Hoechst 33342. Scale bar: 50 µm. c Apoptosis rates were analyzed after treatment with different doses of H₂O₂ (100 and 200 µM) treatment. The apoptosis rates, which were calculated based on at least 100 cells from three experiments, **p < 0.01 versus the untreated cells. d The mRNA expression of ROCK1 in B3 cells under 200 µM H₂O₂ was assessed by quantitative RT-PCR. **p < 0.01 versus the untreated group. e The protein level of ROCK1 increased in lens epithelial cells under 200 µM H₂O₂ treatment. The 100–250 kDa blot was retained by horizontal shear and incubated with the ROCK1 antibody. The 35–50 kDa blot was retained by horizontal shear and incubated with the β-actin antibody