Fig. 8

p53 (ser15) phosphorylation was increased in ROCK1-mediated lens epithelial cells apoptosis in response to H₂O₂ treatment. a The p53 (ser15) phosphorylation level increased in epithelial cells after H₂O₂ treatment for 24 h. The 40–70 kDa blot was retained by horizontal shear and incubated with a p53-p antibody. The 35–50 kDa blot was retained by horizontal shear and incubated with the β-actin antibody. b The knockdown of ROCK1 suppressed the increase in phosphorylation levels of p53 (ser15) in response to H₂O₂. LECs transfected with ROCK1 siRNA were treated with H₂O₂ for 24 h and then lysed for western blotting. Data are based on three independent experiments. The 40–70 kDa blot was retained by horizontal shear and incubated with a p53-p antibody. The 35–50 kDa blot was retained by horizontal shear and incubated with the β-actin antibody. c The p53 (ser15) phosphorylation level increased in B3 cells after transfection with ROCK1 over-expression plasmid. The 40–70 kDa blot was retained by horizontal shear and incubated with a p53-p antibody. The 35–50 kDa blot was retained by horizontal shear and incubated with the β-actin antibody. d Immunohistochemistry showed that the p53 (ser15) phosphorylation level in lens epithelial cells from cataract patient and control group (n = 5 per group). Scale bar: 20 µm. e Western blotting indicated that the p53 (ser15) phosphorylation level increased in lens epithelial of cataract patient compare to the control group. The 40–70 kDa blot was retained by horizontal shear and incubated with a p53-p antibody. The 35–50 kDa blot was retained by horizontal shear and incubated with the β-actin antibody. f Lens sections were immunostained to assess the p53 (ser15) phosphorylation level in the LECs from young and old mice, Scale bar: 20 µm. g Western blotting showed that the p53 (ser15) phosphorylation level was increased in lens epithelial from young and old mice, (n = 5 per group). β-actin was used as an internal reference control. The 40–70 kDa blot was retained by horizontal shear and incubated with a p53-p antibody. The 35–50 kDa blot was retained by horizontal shear and incubated with the β-actin antibody