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Fig. 5 | Molecular Medicine

Fig. 5

From: The involvement of neuroimmune cells in adipose innervation

Fig. 5

Cx3CR1 Cells in Adipose Transit via Lymphatics. Wholemount imaging of female (ae) and male (f) axillary scWAT from Cx3CR1-EGFP reporters mice. Cx3CR1+ cells (green) in lymph node captured by widefield microscopy at 10X (a). Cx3XR1+ cells were shown to occupy the entire depth of the lymph node, captured by confocal microscopy at 20X (b), represented as a z-maximum projection (Glow LUT) (b, left) and 3D reconstruction (depth coded) at two angles (b, middle and right.) Cx3CR1+ cells (green) line lymphatic vasculature (morphologically distinguished from blood vasculature by the bulbous sacs on initial lymphatics, yellow arrow and outlined in white) but are absent in blood vasculature (red arrow) (c). Captured by widefield microscopy at 10X and 40X. Cx3CR1+ cells (Glow LUT) imaged by confocal microscopy at 63X were shown to reside on lymph vessel endothelium and were present within the lumen (d). Z-maximum projection of lymph vessel (d, left) and 3D reconstruction (depth coded) at two angles: looking down z-axis (d, middle) and looking down the x-axis (d, right) with vessel lumen identified by arrow. Tiled z-max projection captured on confocal at 20X demonstrates that Cx3CR1+ cells (white) are found throughout the lymphatic network in scWAT (e). Cx3CR1+ cells (green) reside around nerve bundles marked by the pan-neuronal marker PGP9.5 (magenta) as captured by widefield microscopy at 40X (f)

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