Fig. 2
From: Aerobic exercise improves VCI through circRIMS2/miR-186/BDNF-mediated neuronal apoptosis
Overexpression of circRIMS2 affects the apoptosis of hippocampal neurons. The lentiviral vector pLO-ciR-Control or pLO-ciR-RIMS2 was transfected into HT22 cells for 72 h, then treated with oxygen and glucose deprivation (OGD) for 1 h and grouped: control, OGD, OGD + pLO-ciR-Control, OGD + pLO-ciR-RIMS2. a qRT-PCR detected the expression of circRIMS2 in cells. b qRT-PCR detected the expression of miR-186. c BDNF mRNA level was detected by qRT-PCR, and the BDNF protein level was determined by western blot. d TUNEL detected cell apoptosis. *P < 0.05 versus the control group; **P < 0.01 versus the OGD + pLO-ciR-Control group