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Fig. 3 | Molecular Medicine

Fig. 3

From: Aerobic exercise improves VCI through circRIMS2/miR-186/BDNF-mediated neuronal apoptosis

Fig. 3

The interaction between circRIMS2 and miR-186 and the regulatory effect of miR-186 on BDNF expression. a The luciferase reporter gene test was performed in HEK-293T cells to detect the binding and interaction between circRIMS2 and miR-186. b RNA pull-down detected the binding of circRIMS2 and miR-186. c HT22 cells were transfected with the lentiviral vector pLV-shRNA or pLV-sh-RIMS2 for 48 h, and the expression level of miR-186 was detected by qRT-PCR. d Bioinformatics predicted the binding sites of miR-186 and BDNF, and the luciferase reporter gene test in HEK-293T cells detected the regulatory effect of miR-186 on BDNF. e HT22 cells were transfected with miR-186 mimic or its negative control mimic NC for 48 h, the BDNF mRNA level was detected by qRT-PCR, and the BDNF protein level was determined by western blot. *P < 0.05 versus mimic NC or Bio-NC or pLV-shRNA

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