Fig. 4
From: Aerobic exercise improves VCI through circRIMS2/miR-186/BDNF-mediated neuronal apoptosis
CircRIMS2 inhibits neuronal apoptosis through the miR-186/BDNF pathway. The lentiviral vector pLO-ciR-Control, pLO-ciR-RIMS2, pLO-ciR-RIMS2 + mimic NC, or pLO-ciR-RIMS2 + miR-186 mimic was transfected into HT22 cells for 72 h, then treated with oxygen and glucose deprivation (OGD) for 1 h and grouped: OGD, OGD + pLO-ciR-Control, OGD + pLO-ciR-RIMS2, OGD + pLO-ciR-RIMS2 + mimic NC, OGD + pLO-ciR-RIMS2 + miR-186 mimic. a qRT-PCR detected the expression of circRIMS2 in cells. b qRT-PCR detected the expression of miR-186. c BDNF mRNA level was detected by qRT-PCR, and the BDNF protein level was determined by western blot. d TUNEL detected cell apoptosis. *P < 0.05 versus OGD + pLO-ciR-Control; **P < 0.01 versus OGD + pLO-ciR-RIMS2 + mimic NC