Fig. 5
From: Aerobic exercise improves VCI through circRIMS2/miR-186/BDNF-mediated neuronal apoptosis
Aerobic exercise affects the cognitive function of the VCI model through the circRIMS2/BDNF pathway in vivo. The lentiviral vector pLV-shRNA or pLV-sh-RIMS2 was injected into VCI model mice through lateral ventricle. Repeated ligation of bilateral common carotid arteries (CCA) caused ischemia–reperfusion injury (2VO) to establish a VCI model. The mice were grouped (n = 6 per group): sham, 2VO,2VO + EX,2VO + EX + pLV-shRNA, and 2VO + EX + pLV-sh-RIMS2. a The water maze tested the time that mice found the underwater platform and calculate the average latency. b qRT-PCR was used to detect the expression of circRIMS2 in hippocampus. c qRT-PCR detected the expression of miR-186. d BDNF mRNA level was detected by qRT-PCR, and the BDNF protein level was determined by western blot. e TUNEL detected apoptosis of hippocampal cells. *P < 0.05 versus the sham group; **P < 0.01 versus the 2VO group; #P < 0.05 versus the 2VO + EX + pLV-shRNA group