Fig. 2From: LINC00242/miR-1-3p/G6PD axis regulates Warburg effect and affects gastric cancer proliferation and apoptosisEffects of G6PD silencing on glycolysis in gastric cancer cells. a G6PD knockdown was achieved in AGS and MGC-803 cells by transfecting short hairpin RNA targeting G6PD (sh-G6PD#1 and sh-G6PD#2). The G6PD mRNA expression level was confirmed by qRT-PCR. b, c AGS and MGC-803 cells were transfected with sh-G6PD#1 and sh-G6PD#2 and examined for cell viability by MTT assay (b) and cell apoptosis by Flow Cytometry assay c. d–f Knockdown of G6PD expression significantly decreased glucose consumption (d), lactate production (e) and ATP production (f). N = 3, **P < 0.01 compared to sh-NC groupBack to article page