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Fig. 4 | Molecular Medicine

Fig. 4

From: LINC00242/miR-1-3p/G6PD axis regulates Warburg effect and affects gastric cancer proliferation and apoptosis

Fig. 4

Targeting relationship was identified between G6PD and miR-1-3p. a Three miRNAs (hsa-miR-1-3p, hsa-miR-206 and hsa-miR-613) targeted G6PD in gastric cancer was screened according ENCORI, TargetScan v7.2 and HMDD v3.2 databases. b RT-PCR assay was performed to detect the expression level of these three miRNAs in gastric cancer tissues and paracancerous tissues. N = 77; *P < 0.05, **P < 0.01 compared to adjacent tissues. c The miR-1-3p expression level in gastric cancer cell lines (AGS and MGC-803) and gastric epithelial cell line (GES-1). N = 3; *P < 0.01 compared to GES-1 cell. d Prediction of binding sites of miR-1-3p and G6PD. And the targeting relationship between miR-1-3p and G6PD was verified by luciferase reporter assay in 293 T cell. N = 3; **P < 0.01 compared to mimics NC group; ##P < 0.01 compared to inhibitor NC group. e The effect of miR-1-3p on G6PD mRNA and protein expression in AGS and MGC-803 cells was verified by qRT-PCR and western blot assay. N = 3; **P < 0.01 compared to mimics NC group; ##P < 0.01 compared to inhibitor NC group. f A negative correlation between miR-1-3p and G6PD was verified based on ENCORI databases. g The linear analysis revealed a negative correlation between miR-1-3p and G6PD expression in gastric cancer tissues (N = 77)

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