Fig. 3
From: Aspirin has a better effect on PIK3CA mutant colorectal cancer cells by PI3K/Akt/Raptor pathway
Effect of aspirin on apoptosis of CRC cells a CCK8 assay for detection of apoptosis in CRC cells of different treatment times and different concentrations of aspirin. b JC-1 Assays were used to determine the effect of aspirin at different concentrations on the mitochondrial transmembrane potential of HCT-116 cells. c Flow cytometric analysis for effect of different concentrations of aspirin on apoptosis of HCT-116 cells. d Flow cytometric analysis for effect of different concentrations of aspirin on apoptosis of RKO cells. e The results of Bax and Bcl2 protein expression in differently treated groups in HCT-116 cells. f The results of Bax and Bcl2 protein expression in differently treated groups in RKO cells. g The result of Caspase 3 protein expression in differently treated groups in HCT-116 cells. h The result of Caspase 3 protein expression in differently treated groups in RKO cells. All experiments were repeated 3 times, (n = 3) Mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001 (vs. normal group)