Fig. 5From: Exosomal miR-335 derived from mature dendritic cells enhanced mesenchymal stem cell-mediated bone regeneration of bone defects in athymic ratsmDC-Exo enhanced BM-MSCs-mediated bone regeneration by transferring miR-335. Rats were randomly divided into five groups: Matrix, Matrixā+āBM-MSCs, Matrixā+āBM-MSCsā+āmDC-Exo, Matrixā+āBM-MSCsā+āmDC-Exo-NC, and Matrixā+āBM-MSCsā+āmDC-Exo-miR-335I. a Bone regeneration was monitored via micro-CT scanning at 4, 8 and 12Ā weeks post-surgery. b Quantification of new bone volume from micro-CT scanning. c Representative H&E staining showing bone defect repair of posterior limbs of rats. d Quantification of new bone area from H&E staining. The protein levels of e ALP, Runx2, and f, g LAST1 in the bone examined by western blot. *Pā<ā0.05, **Pā<ā0.01, vs. Matrix; #Pā<ā0.05, ##Pā<ā0.01, vs. Matrixā+āBM-MSCs; $Pā<ā0.05, $$Pā<ā0.01, vs. Matrixā+āBM-MSCsā+āmDC-Exo-NCBack to article page