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Fig. 4 | Molecular Medicine

Fig. 4

From: ELAVL1 is transcriptionally activated by FOXC1 and promotes ferroptosis in myocardial ischemia/reperfusion injury by regulating autophagy

Fig. 4

Inhibition of autophagy suppresses H/R induced ferroptosis and cell injury. Myocardial cells were pretreated with 3-MA (5 mM) for 24 h before being subjected to H/R. a, b Relative protein levels of Beclin-1, p62 and LC3 were determined by western blotting. c–e Relative mRNA and protein levels of GPx4 in cells were assessed respectively. g CCK8 assay was performed to assess cell viability. (G&H) Relative cellular ROS levels in 3-MA treated cells were measured after H/R. Relative cellular iron level (i), LDH activity (j), LPO level (k) and GSH level (l) in each group of cells were detected by corresponding assay kits after H/R. The results were presented as the mean ± SD. n = 3; *P < 0.05, **P < 0.01, ***P < 0.001

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