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Fig. 2 | Molecular Medicine

Fig. 2

From: Long non-coding RNA CIR inhibits chondrogenic differentiation of mesenchymal stem cells by epigenetically suppressing ATOH8 via methyltransferase EZH2

Fig. 2

LncRNA CIR knockdown promotes chondrogenic differentiation of hUC-MSCs. a qRT-PCR analysis of lncRNA CIR level in control and differentiated hUC-MSCs. b qRT-PCR analysis of ATOH8 mRNA level in control and differentiated hUC-MSCs. c Western blot analysis of ATOH8 protein level in control and differentiated hUC-MSCs. d qRT-PCR analysis of lncRNA CIR level in different transfected hUC-MSCs. e Alcian blue staining to analyze chondrogenic differentiation of hUC-MSCs transfected with different constructs. f Western blot analysis of levels of chondrogenic markers including SOX9, Aggrecan, and Col2A1 in transfected cells. g Immunostaining analysis of levels of Col2A1 and Aggrecan in transfected cells. n = 3; *P < 0.05; **P < 0.01; ***P < 0.001

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