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Fig. 3 | Molecular Medicine

Fig. 3

From: Long non-coding RNA CIR inhibits chondrogenic differentiation of mesenchymal stem cells by epigenetically suppressing ATOH8 via methyltransferase EZH2

Fig. 3

Overexpression of ATOH8 promotes chondrogenic differentiation of hUC-MSCs. a qRT-PCR analysis of ATOH8 level in transfected cells. b Western blot analysis of ATOH8 protein level in transfected cells. c Alcian blue staining to analyze chondrogenic differentiation of hUC-MSCs transfected with different constructs. d Western blot analysis of expression levels of SOX9, Aggrecan, and Col2A1 in transfected cells. e Immunostaining analysis of levels of Col2A1 and Aggrecan in transfected cells. n = 3; * P < 0.05; **P < 0.01; ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658