Fig. 2

CircROBO2 played a biological role through miR-1184. a Circ Interactome predicted the putative targeting sites for circROBO2 and miR-1184. b Luciferase activity analysis. c The RIP method was used in primary cardiomyocytes. Determine the degree of enrichment of circROBO2 and miR-1184 RNA in the IP complex. Anti-immunoglobulin G (IgG) was used as a control. d Knockdown or overexpression of circROBO2 or miR-1184 in primary cardiomyocytes. e The expression levels of miR-1184 in ischemic myocardium and normal tissues. f The expression levels of miR-1184 in myocardial cells of mice exposed to normoxic or hypoxic conditions. All above the experiments were repeated three times. *P < 0.05, **P < 0.01, ***P < 0.001