Fig. 3

CircROBO2 up-regulated the expression of TRADD by sponging miR-1184. a Putative binding sites for miR-1184 and TRADD3′-UTR. b Analysis of luciferase activity of PGL-miR-1184 in HEK293 cells by co-transfecting withTRADD-3′-UTR-WT or TRADD-3′-UTR-Mut vector. The experiments were repeated three times. P < 0.05, **P < 0.01, ***P < 0.001. c TRADD mRNA expression levels were detected by co-transfecting miR-1184 mimics or miR-NC, circROBO2 vector or control vector in primary cardiomyocytes. The experiments were repeated three times. P < 0.05, **P < 0.01, ***P < 0.001. d Expression levels of TRADD protein in primary myocardial cells after co-transfecting with miR-1184 mimics, miR-NC + circROBO2 vector or miR-1184 + circROBO2. The experiments were repeated three times. e IHC analysis of caspase3 in myocardium form sham and MI group. f Expression levels of TRADD protein in cardiomyocytes of mice exposed to normoxic or hypoxic conditions (12, 24 and 48 h). There mice were used for this experiment. * vs control group, # vs circROBO2 group. **P < 0.01, ^##P < 0.01