Table 1 The sequences of specific primers
Gene name | Primer sequence (5′ to 3′) |
|---|---|
XIST | Forward: 5′-ACG CTG CAT GTG TCC TTA G-3′ Reverse: 5′-GAG CCT CTT ATA GCT GTT TG-3′ |
miR-192 | Forward: 5′-CTGACCTATGAATTGACAGCCA-3′ Reverse: 5′-GCTGTCAACGATACGCTACGT-3′ |
TRIM25 | Forward: 5′-GTCTCTACC CAGA ACAGTTTCC-3′ Reverse: 5′-ATCCAACACAGGCTGATTCC-3′ |
GAPDH | Forward: 5′-CACCCACTCCTCCACCTTTG-3′ Reverse: 5′-CCACCACC CTGTTGCTGTAG-3′ |
U6 | Forward: 5′-TGCGGGTGCTCG CTT CGG CAGC-3′ Reverse: 5′-CCAGTGCAGGGTCCGAGGT-3′ |