Fig. 2

MaR1 reduces hepatocyte apoptosis during hepatic I/R injury. a Confocal images of TMR (red), nucleus (blue), and F-actin (green) in liver sections obtained from PBS or MaR1-treated mice subjected to sham surgery or 1 h ischemia/6 h reperfusion (scale bar = 25 μm). b Percentage of TMR-positive cells per liver section (n = 3 or 4/gp). c Western blot analysis of apoptosis markers (Bax, Bcl-2 and cleaved-caspase3) in liver tissues from PBS- and MaR1-treated mice that underwent sham operation or liver I/R insult (1 h/6 h). d LDH release in primary mouse HCs, NPCs, or HC/NPC co-cultures subjected to normoxia or 10 h hypoxia (1% O2)/10 h reoxia (H/R 10 h/10 h) without (0) or with pretreatment with 30 or 100 nM MaR1. e Western blot analysis of Bcl-2, Bax and cleaved-caspase3 in primary mouse HCs treated with normoxia or H/R without (0) or with 100 nM MaR1 given prior to H/R. Images representative of at least 3 separate experiments. All data are shown as mean ± SEM. *P\(<\)0.05