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Fig. 1 | Molecular Medicine

Fig. 1

From: miR-325-3p, a novel regulator of osteoclastogenesis in osteolysis of colorectal cancer through targeting S100A4

Fig. 1

miR-325-3p downregulates in osteoclast precursors in bone metastasis of CRC. a 100,000 CT-26 cells were injected into tibias in mice and the samples were collected at specific timepoints to be sectioned. Safranin O-Fast Green staining revealed trabecular area (Scar bar = 50 μm) (n = 3 mice). b Quantification of trabecular area in (a) (mean ± SE). c TRAP staining revealed TRAP positive cells in trabecular area at specific timepoints after injection of CT-26 cells (Scar bar = 50 μm) (n = 3 mice). d Quantification of relative area of osteoclast surface in (c) (mean ± SE). e Primary OCPs were isolated from bone marrow at specific timepoints through FACS. Real-time PCR analysis showed the relative expression of miR-325-3p in OCPs at 14 d.p.i comparing with OCPs at 0 d.p.i. (D0, n = 3 mice; D14, n = 5 mice) (mean ± SD). f. Primary OCPs were isolated and cultured in DMEM or CT-26 CM for 4 days in the presence of RANKL and M-CSF. Real-time PCR analysis showed the relative expression of miR-325-3p in OCPs treated by CM or DMEM (mean ± SD). In vitro experiments were performed at least 3 times. *p < 0.05, **p < 0.01, ***p < 0.001

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