Fig. 2

miR-325-3p regulates CRC stimulated osteoclastogenesis. a OCPs were isolated through FACS and maintained in DEME or CT-26 CM and transfected with agomir-325-3p, antagomir-325-3p or negative controls in the presence of RANKL and M-CSF for 4 days. TRAP positive multinucleated cells with more than three nuclei were counted as mature osteoclasts (Scar bar = 10 μm). b Quantification of osteoclast number in each group in (a) (mean ± SE). c Relative TRAP activity assay indicated the TRAP activity changes in each group in (a) (mean ± SE). d Real-time PCR analysis showed the mRNA levels of Cathepsin K in each group in (a) (mean ± SD). e Real-time PCR analysis showed the mRNA level of TRAP in each group in (a) (mean ± SD). In vitro experiments were performed at least 3 times. *p < 0.05, **p < 0.01, ***p < 0.001