Fig. 1
From: TGF-β-induced α-SMA expression is mediated by C/EBPβ acetylation in human alveolar epithelial cells
TGF-β induced EMT and deposition of collagen-I in A549 cells. a A549 cells were exposed to TGF-β (0−20 ng/mL) for 24 h. Protein levels of α-SMA and E-cadherin were determined using western blotting, and quantification of western blots were calculated according to grey analysis. In agreement with the elevated expression of α-SMA, E-cadherin expression decreased in a dose-dependent manner, compared to non-stimulated cells. b Expression of α-SMA and E-cadherin in A549 cells treated with TGF-β (10 ng/mL) for 0, 6, 12, 24, and 48 h was assessed using western blotting. c, d Quantification of western blots were calculated according to analysis. e ELISA analysis of collagen-I levels in the supernatant of A549 treated with 10 ng/mL TGF-β for 24 h or controls. All data were from three separate experiments. Asterisk (*) denotes significant difference (P < 0.05)