Fig. 3
From: TGF-β-induced α-SMA expression is mediated by C/EBPβ acetylation in human alveolar epithelial cells
TGF-β up-regulated the expression of α-SMA via C/EBPβ activation in A549 cells. a TGF-β increased α-SMA protein levels via C/EBPβ activation. A549 cells were treated with TGF-β (10 ng/mL) for 24 h with or without the presence of C/EBPβ siRNA (10 nM) for 8 h. Levels of C/EBPβ and α-SMA in cell lysate were analysed using western blotting. b Quantification of western blots were calculated according to grey analysis. c The C/EBPβ siRNA blocked TGF-β-induced increase in collagen-I synthesis. The effect of C/EBPβ siRNA on collagen-I deposition in TGF-β-treated A549 cells was analysed using ELISA. d TGF-β-induced C/EBPβ-luciferase activity is regulated by the C/EBPβ siRNA. A549 cells transfected with C/EBPβ siRNA successfully attenuated TGF-β-activated C/EBPβ-luciferase activity. Asterisk (*) denotes significant difference (P < 0.05)