Fig. 4
From: TGF-β-induced α-SMA expression is mediated by C/EBPβ acetylation in human alveolar epithelial cells
TGF-β induced C/EBPβ binding to α-SMA promoter in A549 cells. a The 5′ promoter sequence of the human, mouse and rat α-SMA gene contains a putative C/EBPβ-binding motif (TTGGGCAA) within 200 bp from the transcription start site. *Indicates conserved sequences. b A549 cells were exposed to TGF-β (10 ng/mL) for 3 h, followed by ChIP assay. ChIP primer pairs, yielding 237-bp PCR products, were designed to amplify DNA corresponding to the C/EBPβ-binding site. A rabbit IgG clone was used as a negative control. Typical traces are representative of two experiments with similar results