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Fig. 3 | Molecular Medicine

Fig. 3

From: Essential properties and pitfalls of colorimetric Reverse Transcription Loop-mediated Isothermal Amplification as a point-of-care test for SARS-CoV-2 diagnosis

Fig. 3

RT-LAMP internal control results. a Colorimetric readout after 30 min of 18S internal control RT-LAMP reaction. Samples were pipette in a 384-well plate and the optical density was measured at 434 nm and 560 nm using a BioteK Synergy reader. To obtain the ΔOD (color change difference), absorbance at 560 nm was subtracted from the one at 434 nm. The line inside the box indicates the median and the whiskers extend either to the minimum and maximum values. * Indicates difference (p > 0.001) between groups (unpaired, t-test). b Detection of internal control 18S by RT-LAMP assay. Comparison between colorimetric RT-LAMP results and RT-qPCR CT values (RNAse P)

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