Fig. 3

The in vitro effect of Btc recombinant protein on Schwann cell migration. a Representative Transwell migration images and normalized averaged migration rate of Schwann cells treated with or without Btc recombinant protein. Scale bars represented 50 μm. Transwell migration assay was done three times using duplicate or triplicate wells. Statistical evaluation was performed using Mann–Whitney test. *p-value < 0.05 versus non-treated cells. b Representative wound healing images and percentage decrease in scratch of Schwann cells treated with or without Btc recombinant protein. Scale bars represented 100 μm. Transwell migration assay was done three times using duplicate or triplicate wells. Statistical evaluation was performed using Mann–Whitney test. *p-value < 0.05 versus non-treated cells