Fig. 5

The in vivo effect of Btc recombinant protein after rat sciatic nerve crush. Representative immunofluorescence images of rat sciatic nerve segments treated with saline control or Btc recombinant protein at 4 days after nerve crush. Averaged intensity of SCG10 at 1, 2, 3, 4, 5, and 6 mm from the crush site were normalized to its intensity at the crush site. Scale bar indicated 1000 µm. Axon length was summarized from three experiments. Statistical evaluation was performed using multiple t-test. *p-value < 0.05 versus saline control