Fig. 1

Overexpression of miR-34a decreases oncogenic signaling in glioblastoma cells. a Representative western blot images showing reduction in phosphorylated Akt, ERK and Rb and increased p21 expression in miR-34a-transfected relative to control miRNA-transfected GBM6 and A172 glioblastoma cells. Cell lysates were prepared 2 days post-transfection and analyzed by western blotting. b Western blot data quantification (% reduction in luminescence relative to GAPDH) in the A172 cell line. Histograms showing means (± SD) of luminescence signals from three independent experiments, normalized to that of GAPDH. A paired t-test was used to test for significant difference between mean luminescence values. *p < 0.05