Table 2 Exosomal miRNAs in tuberculosis
Exosomal miRNA | Source | Conclusion | Method for exosomes | Refs |
|---|---|---|---|---|
miR-484, miR-425, and miR-96 | Serum | The findings of this research indicate that exosomal miRNAs have a potential capacity for the diagnosis of active TB | Total exosome isolation (TEI) reagent (Invitrogen, Thermo Fisher Scientific Corporation, USA) | Mortaz et al. (2019) |
miR-20a, miR-20b, miR-26a, miR-106a, miR-191, miR-486 | Plasma | This study's results have indicated that the combination of exosomal miRNAs and EHRs might enhance the medical diagnosis of TBM and PTB | ExoQuick Kit (System Biosciences, USA) | Hu et al. (2019) |
hsa-let-7e-5p, hsa-let-7d-5p, hsa-miR-450a-5p, and hsa-miR-140-5p, hsa-miR-1246, hsa-miR-2110, hsa-miR-370-3P, hsa-miR-28-3p, and hsa-miR-193b-5p | Serum | This research's outcomes recommended that exosomal miRNA use could promote novel molecular targets to distinguish LTBI and active TB | Differential centrifugation, filteration, and ultrafiltration | Lyu et al. (2019) |
hsa-miR-140-3p, hsa-miR-3184-5p and hsa-miR-423-3p | Serum | The study’s findings imply the preferential packing of RNA loads for exosomes at various steps of Mtb infection and promote further analysis and production of TB pathogenesis | Differential centrifugation, filteration, and ultrafiltration | Lyu et al. (2018) |
miR-1224, -1293, -425, -4467, -4732, -484, -5094, -6848–6849, -4488 and -96 | PBMCs | This study's findings offer support for the release of unique exosomal miRNAs from BCG-infected MDMs, which highlighted the interaction between host and pathogen following infection | Total exosome isolation (TEI) reagent (Invitrogen by the Thermo Fisher Scientific Corporation, Waltham, MA, USA) | Alipoor et al. (2017) |
Over 100 transcripts were found, such as 99b-5p, Mmu 30c, Mmu 30a, Mmu 191, Mmu 378, Mmu 210, Mmu 423-5p and Mmu 486-5p | Cell culture, Mtb infected RAW264.7 macrophages | This study has shown that there is discriminating packing of RNA material in exosomes after Mtb infection | Centrifugetion, filtration and linear sucrose gradient | Singh et al. (2015a) |
miR-205-5p, miR-483-5p, miR-375, miR-200c-3p, miR-429, miR-200b-3p, miR-200a-3p, miR-203a-3p, and miR-141-3p | Pleural effusion | This study's outcomes have revealed that distinct miRNA profiles could be promising as biomarkers for differential determination of PEs with further confirmation dependent on larger cohorts | Differential centrifugation, filteration, and ultrafiltration | Wang et al. (2017) |