Fig. 6

EA inhibited the NF-κB signalling pathway via the upregulation of OTULIN expression. a Western blotting was used to detect OTULIN, p-IκBα, IκBα, and cytoplasm/nucleus-p65 proteins. β-Actin served as a loading control for total or cytoplasmic protein, and Lamin B served as a nuclear protein. b, n = 3) Quantitative analysis of OTULIN protein, the phosphorylation ratio of IκBα, and nuclear/cytoplasmic NF-κB p65. c, n = 6) Immunohistochemistry was performed to detect IκBα and NF-κB p65 proteins. Red arrows indicate IκBα- or NF-κB p65-positive cells. Scale bar = 100 μm. &&&P < 0.001, &&P < 0.01 and &P < 0.05 versus the tMCAO group; $$$P < 0.001 versus the tMCAO + EA group; ###P < 0.001 versus the tMCAO + EA + LV-Scramble group