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Fig. 7 | Molecular Medicine

Fig. 7

From: OTULIN is a new target of EA treatment in the alleviation of brain injury and glial cell activation via suppression of the NF-κB signalling pathway in acute ischaemic stroke rats

Fig. 7

OTULIN silencing weakened the inhibitory effect of EA on microglial activation in focal cerebral ischaemia/reperfusion rats. A Representative images of Iba-1+ cells in the ischaemic penumbra of the cerebral cortex: Aa Ramified cells; Ab Internal cells; Ac Ameboid cells. The Iba-1+ microglial cells in the ischaemic penumbra of cerebral ischaemia were detected using immunofluorescence 24 h (b) and 72 h (e) after reperfusion. Histograms c (n = 6) and f (n = 6) present the quantitative analyses of mean Iba-1 immunofluorescence intensity at 24 h and 72 h, respectively. Histograms d (n = 6) and g (n = 6) present the quantitative analyses of the proportions of ramified, internal, and ameboid cells at 24 h and 72 h, respectively. The contents of TNF-α (h, n = 6), IL-1β (i, n = 6) and IL-6 (j, n = 6) at 24 h and 72 h were detected. Scale bar = 50 μm. ***P < 0.001 and *P < 0.05 versus the Sham group; &&&P < 0.001, &&P < 0.01, and &P < 0.05 versus the tMCAO group; $$$P < 0.001, $$P < 0.01 and $P < 0.05 versus the tMCAO + EA group; ###P < 0.001, ##P < 0.01, and #P < 0.05 versus the tMCAO + EA + LV-Scramble group

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