Fig. 3

CDK1 mediates ISL1 phosphorylation at serine 269. a MGC803 cells transfected with plasmid encoding CDK1 or control vector (left), negative control or CDK1 si-RNA (right). Lysate was analyzed by immunoblotting with antibodies against the indicated proteins. b MGC803 cells were transfected with plasmids encoding dominant‑negative mutant CDK1 (DN‑CDK1) or CDK1, and lysate was analyzed by immunoblotting with antibodies against the indicated proteins. c MKN28 cells expressing wild-type ISL1 or ISL1-S269A were transfected with plasmid encoding CDK1 or control vector. Cell lysates were immunoblotted with antibodies against the indicated proteins. d MKN28 cells were transfected with plasmids encoding wild-type ISL1 or ISL1-S269A and treated with CDK1 inhibitor RO-3306 or DMSO. Lysates were analyzed by immunoblotting with antibodies against the indicated proteins. GAPDH served as the internal control. e Representative IHC staining of ISL1-S269-p expression in GC (right, n = 60) and paired adjacent tissues (left, n = 60). f Relationship between expression of ISL1-S629-p and overall survival in gastric cancer