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Fig. 4 | Molecular Medicine

Fig. 4

From: Phosphorylation of islet-1 serine 269 by CDK1 increases its transcriptional activity and promotes cell proliferation in gastric cancer

Fig. 4

Phosphorylated ISL1 showed improved localization on cyclinB1 and cyclinB2 promoters. a ChIP assay analysis and PCR gel electrophoresis analysis of ISL-1 recruitment to the cyclinB1 and cyclinB2 promoters in MGC803 cells. b ChIP-re-IP assay was performed with anti-ISL-1 or rabbit IgG antibodies and then with anti-CDK1 or IgG antibodies for immunoprecipitation using chromatin harvested from MGC803 cells. c, d Quantitative chromatin immunoprecipitation analysis of cyclinB1 and cyclinB2 promoters in MKN28 cell line transfected with control vector or expressing wild-type ISL1 or ISL1-S269A plasmids. Data are expressed as mean ± s. d. from three individual experiments. (*p < 0.05, WT vs. control, #p < 0.05, S269A vs. control, $p < 0.05, WT vs. S269A)

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Molecular Medicine

ISSN: 1528-3658