Skip to main content
Fig. 5 | Molecular Medicine

Fig. 5

From: Phosphorylation of islet-1 serine 269 by CDK1 increases its transcriptional activity and promotes cell proliferation in gastric cancer

Fig. 5

Phosphorylation Ser 269 of ISL1 increases transactivation potency. a, b Effect of CDK1 activity on the transcriptional activity of wild-type ISL1 or S269A ISL1. Transcriptional activity of wild-type ISL1 and S269A ISL1 on cyclinB1-luc and cyclinB2-luc determined by luciferase reporter assay in MKN28 cells. c Cell proliferation was studied by CCK-8 analysis of MKN28 cells transfected with plasmids encoding control vector and wild-type ISL1 or ISL1-S269A. d Cell cycle distributions were analyzed by flow cytometry in MKN28 transfected with plasmids encoding control vector and wild-type ISL1 or ISL1-S269A. The data represent 3 independent experiments, each performed in triplicate. Each bar represents mean ± SD. (*p < 0.05, WT vs. control, #p < 0.05, S269A vs. control, $p < 0.05, WT vs. S269A)

Back to article page

Molecular Medicine

ISSN: 1528-3658