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Fig. 6 | Molecular Medicine

Fig. 6

From: Phosphorylation of islet-1 serine 269 by CDK1 increases its transcriptional activity and promotes cell proliferation in gastric cancer

Fig. 6

Phosphorylation of ISL1 increases self-stability but does not increase its interaction with CDK1. a NIH3T3 cells were transfected with wild-type ISL1 or ISL1-S269A plasmids. After 48 h, cells were treated with CHX (100 µg/ml) for 12 h. Whole cell extracts were harvested for western blotting. b Co-IP assays were performed in NIH3T3 cells to detect the interaction between ISL1 (wild-type or mutants) and CDK1. GAPDH served as a loading and negative control. c Schematic representation of positive feedback regulation by the ISL1-CDK1/cyclin B1/2 complex in GC. Full arrow: confirmed; broken arrow: to be verified

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Molecular Medicine

ISSN: 1528-3658