Fig. 1
From: MiR-27a-3p promotes the osteogenic differentiation by activating CRY2/ERK1/2 axis
Expression of miR-27a-3p and CRY2 were detected in MC3T3-E1 cells. MC3T3-E1 cells were cultured with OIM. a Alizarin Red staining in MC3T3-E1 cells. b Detection of ALP activity. c The expression of OCN, OPN and RUNX2 were measured by western blotting. d, e RT-qPCR tested miR-27a-3p and CRY2 levels. f Western blotting analyzed the level of ERK1/2. *P < 0.05, **P < 0.01, ***P < 0.001