Fig. 2
From: MiR-27a-3p promotes the osteogenic differentiation by activating CRY2/ERK1/2 axis
Knockdown of miR-27a-3p and overexpression of CRY2 inhibited ODM. MC3T3-E1 cells were transfected with miR-27a-3p inhibitor or CRY2 overexpression. a RT-qPCR detected the levels of miR-27a-3p. b RT-qPCR and western blotting examined CRY2 expression. c Alizarin Red staining in MC3T3-E1 cells. d Detection of ALP activity. e Western blotting analyzed the expression of OCN, OPN and RUNX2. *P < 0.05, **P < 0.01, ***P < 0.001