Fig. 3
From: MiR-27a-3p promotes the osteogenic differentiation by activating CRY2/ERK1/2 axis
CRY2 was a direct target of miR-27a-3p. a RT-qPCR examined the expression of miR-27a-3p. b, c RT-qPCR and western blotting detected CRY2 expression. d Dual luciferase report analysis the interaction of miR-27a-3p and CRY2. e Western blotting analyzed ERK1/2 level. f MC3T3-E1 cells were transfected with CRY2 shRNA. The protein expressions of Akt, p-Akt, ERK, p-ERK and BMAL2 in MC3T3-E1 cells were detected by western blotting. *P < 0.05, **P < 0.01, ***P < 0.001