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Fig. 5 | Molecular Medicine

Fig. 5

From: MiR-27a-3p promotes the osteogenic differentiation by activating CRY2/ERK1/2 axis

Fig. 5

CRY2 knockdown could reverse the effect of miR-27a-3p inhibitor on cell apoptosis and autophagy. Cells were treated with miR-27a-3p inhibitor, miR-27a-3p inhibitor + CRY2 shRNA or miR-27a-3p inhibitor + honokiol. a The apoptosis of cells was tested by flow cytometry. b The protein expressions of Bax, Bcl-2 and cleaved caspase 3 were detected by western blotting. c The protein expressions of LC3 I and LC3 II were detected by western blotting. *P < 0.05, **P < 0.01, ***P < 0.001

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