Fig. 4

RNA-seq analysis revealed a role of Rab7b in EAE. a A heatmap showing DEGs in spinal cord of WT mice versus LRRC4^−/− mice from cluster analysis of RNA-seq. b The top 10 GO terms of up-regulated and down-regulated DEGs between WT and LRRC4^−/− mice. c The top 10 KEGG pathways of up-regulated and down-regulated DEGs between WT and LRRC4^−/− mice. d The comparison of GO terms between up-regulated DEGs and down-regulated DEGs. e Comparison of KEGG pathways between up-regulated DEGs and down-regulated DEGs. f Rab7b mRNA levels of spinal cords in WT and LRRC4^−/− mice as determined with real-time PCR. Results are shown as means ± SEM (n = 4). g Rab7b protein levels of spinal cords in WT and LRRC4^−/− mice as assessed with western blotting. h Rab7b mRNA levels of the spinal cords in healthy and EAE mice assessed with real-time PCR. Results are shown as means ± SEM (n = 4). i Correlation analysis of Rab7b and LRRC4 mRNA levels in healthy and EAE mice. *p < 0.05, **p < 0.01