Fig. 5

CircPTK2 directly targeted miR-181c-5p and was negatively correlated with miR-181c-5p in LPS-induced microglia. a After treatment with RNase R, PTK2 level decreased significantly, while CircPTK2 level remained unchanged. N = 3 for each column; ^△△△P < 0.001 t test vs Rnase R-. b The results from starBase v2.0 (http://starbase.sysu.edu.cn/starbase2/) and c dual luciferase assay showed that circPTK2 could target miR-181c-5p. N = 3 for each column; ^‡‡‡P < 0.001 t test vs MC. d After induction with different doses of LPS, qRT-PCR was used to detect the expressions of miR-181c-5p and circPTK2 in microglia. β-actin or U6 was used as a control. N = 3. Each experiment was repeated three times independently. M miR-181c-5p mimic; MC miR-181c-5p mimic control; qRT-PCR quantitative reverse transcription real time polymerase chain reaction