Fig. 7

Effects of circPTK2 and miR-181c-5p on learning and memory functions, mitochondrial function, hippocampal cell apoptosis and inflammation in CLP-induced mice. a and b Morris water maze test was used to measure the latency period and all quadrant residence time of mice in the Sham, CLP, CLP + siNC, CLP + sicirc, CLP + sicirc + antagomir groups. N = 6 per group; *P < 0.05, **P < 0.01, ***P < 0.001 vs. Sham; ^{^}P < 0.05, ^{^^}P < 0.01 vs. CLP + siNC^{; #}P < 0.05, ^##P < 0.01 vs. CLP + siCirc; One-way ANOVA followed by Bonferroni's post-hoc test. c CircPTK2 knockdown partially restored mitochondrial membrane potential in CLP-induced mice, and miR-181c-5p antagomir again reversed the effect of sicircPTK2. N = 3 for each column; ***P < 0.001 vs. Sham; ^{^^^}P < 0.001 vs. CLP + siNC; ^###P < 0.001 vs. CLP + siCirc; One-way ANOVA followed by Bonferroni's post-hoc test. d The effects of circPTK2 and miR-181c-5p on hippocampal cell apoptosis was determined by TUNEL. N = 3 for each column; ***P < 0.001 vs. Sham; ^{^^^}P < 0.001 vs. CLP + siNC; ^###P < 0.001 vs. CLP + siCirc; One-way ANOVA followed by Bonferroni's post-hoc test. e–g The expressions of IL-1β, TNF-α and Caspase-3 in the hippocampus of mice in each group were detected by qRT-PCR and Western blot. N = 3 for each column; ***P < 0.001 vs. Sham; ^{^^^}P < 0.001 vs. CLP + siNC; ^###P < 0.001 vs. CLP + siCirc; One-way ANOVA followed by Bonferroni's post-hoc test. CLP Cecal Ligation and Puncture; Sicirc sicircPTK2; qRT-PCR quantitative reverse transcription real time polymerase chain reaction. β-actin were used as control