Fig. 4
From: Redox modifications of cysteine residues regulate the cytokine activity of HMGB1
Redox-dependent effect on HMGB1-induced NF-κB activation in primary human macrophages. Analysis of phosphorylated NF-κB p65 subunit following the stimulation of cultured macrophages in 6-well plate for 1 h with HMGB1 (5 µg/mL) or LPS (4 ng/mL). HMGB1 was exposed to either H2O2 (50 mM, 120 min) or DTT (5 mM, 120 min) prior to the assay. LPS was exposed to DTT (5 mM, 120 min). Expression of phosphorylated NF-κB p65 and total NF-κB p65 (loading control) was measured by quantitative ELISA. Data are presented as ratio of phospho-NF-κB vs. total NF-κB or means ± SEM. n = 3. *p < 0.05. One-way ANOVA followed by Tukey’s multiple comparisons test between groups