Fig. 4

The HSP72 inhibitor CCT251236 inhibited the therapeutic effect of baicalin on COPD in vivo. a HSP72 expression in the lung tissues of mice was tested by immunohistochemistry (×400). b Lung injury was visualized by H&E staining (×400) and lung injury was scored. c The white blood cells in the BALF were measured by ELISA. d Muc5AC expression in the BALF was determined. e Expression of IL-6, IL-8, TNF-α in the BALF was measured by ELISA. Compared with the control group, *p < 0.05, **p < 0.01, ***p < 0.001; compared with the CS + LPS group, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001; compared with the CS + LPS + baicalin + vehicle group, ^&p < 0.05, ^&&p < 0.01, ^&&& p < 0.001. All experiments were repeated for three times independently and the data were represented as mean ± standard deviation. One-way ANOVA followed by Tukey’s test was used for multiple group comparison