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Fig. 5 | Molecular Medicine

Fig. 5

From: Baicalin alleviates chronic obstructive pulmonary disease through regulation of HSP72-mediated JNK pathway

Fig. 5

HSP72 inhibits COPD through the inhibition of the JNK signaling pathway. a MLE-12 cells were transfected with si-HSP72 and the expression of HSP72 and JNK phosphorylation normalized to GAPDH was analyzed by immunoblotting; *p < 0.05, **p < 0.01, ***p < 0.001 compared to cells transfected with si-NC. b The extent of JNK phosphorylation normalized to GAPDH after SP600125 treatment was analyzed by immunoblotting; *p < 0.05, **p < 0.01, ***p < 0.001 compared to cells treated with DMSO. MLE-12 cells were treated with SP600125 and stimulated with CSE. c The phosphorylation of JNK normalized to GAPDH was analyzed by immunoblotting. d Cell viability was detected by CCK-8 assay. e Cell apoptosis was detected by flow cytometry. f Release of IL-6, IL-8, and TNF-α was analyzed by ELISA. *p < 0.05, **p < 0.01, ***p < 0.001 compared to untreated cells; #p < 0.05, ##p < 0.01, ###p < 0.001 compared to cells treated with CSE. MLE-12 cells were co-treated with si-HSP72 and SP600125. g Cell viability was detected by CCK-8 assay. h Cell apoptosis was detected by flow cytometry. i Release of IL-6, IL-8, and TNF-α was analyzed by ELISA. *p < 0.05, **p < 0.01,***p < 0.001 compared to CSE + SP600125. All experiments were repeated for three times independently and the data were represented as mean ± standard deviation. One-way ANOVA followed by Tukey’s test was used for multiple group comparison

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