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Fig. 3 | Molecular Medicine

Fig. 3

From: LncRNA SOX2OT alleviates mesangial cell proliferation and fibrosis in diabetic nephropathy via Akt/mTOR-mediated autophagy

Fig. 3

SOX2OT overexpression inhibited mesangial cell proliferation and fibrosis. Mouse mesangial cells were incubated with high glucose (HG) or normal glucose (NG). HG cells were transfected with pcDNA-SOX2OT or pcDNA-vector. a mRNA expression of SOX2OT was detected by RT-qPCR in HG cells after SOX2OT overexpression. b Cell survival was measured using CCK-8 assay after SOX2OT overexpression. c EDU assay was performed to assess proliferation in HG cells after SOX2OT overexpression. d Western blot analysis of TGFβ-1, fibronectin, ASK1 and collagen IV in HG cells after SOX2OT overexpression. e Western blot analysis of E-cadherin and N-cadherin in HG cells after SOX2OT overexpression. GAPDH was used as a loading control in western blot analysis. Error bars represent the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, One-way-ANOVA followed by Newman-Keuls post hoc test

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Molecular Medicine

ISSN: 1528-3658